Type of culture medium is associated with preimplantation embryo development
نویسندگان
چکیده
منابع مشابه
Mitochondrial DNA content in embryo culture medium is significantly associated with human embryo fragmentation.
STUDY QUESTION Is the amount of cell-free DNA released by human embryos into culture medium correlated with embryo morphological features? SUMMARY ANSWER The mitochondrial DNA (mtDNA) content of culture medium is significantly associated with the fragmentation rate on Days 2 and 3 of embryo development, whether the oocyte came from women ≤ 35 or >35 years old. WHAT IS KNOWN ALREADY Cellular...
متن کاملHUWE1 plays important role in mouse preimplantation embryo development and the dysregulation is associated with poor embryo development in humans
HUWE1 is a HECT domain containing ubiquitin ligase implicated in neurogenesis, spermatogenesis and cancer development. The purpose of the current study is to investigate the role of HUWE1 in early embryo development. Here we demonstrate that Huwe1 is expressed in both nucleus and cytoplasm of preimplantation mouse embryos as well as gametes. Hypoxia (5% O2) treatment could significantly increas...
متن کاملP-127: The Effect of Supplementation of In Vitro Culture Medium with FGF2 on Ovine Embryo Development
Background: Fibroblast growth factors are a great family of growth factors that exert variety of functions in vertebrates including cell growth, survival, differentiation and migration. FGFRs are transmembrane tyrosine kinase receptors transmitting FGF signals. Four types of FGFRs (1/2/3/4) exist in vertebrates. FGF2 is one of the FGFs detected in bovine and ovine uterus flushes, supposing that...
متن کاملFAM deubiquitylating enzyme is essential for preimplantation mouse embryo development
FAM is a developmentally regulated substrate-specific deubiquitylating enzyme. It binds the cell adhesion and signalling molecules beta-catenin and AF-6 in vitro, and stabilises both in mammalian cell culture. To determine if FAM is required at the earliest stages of mouse development we examined its expression and function in preimplantation mouse embryos. FAM is expressed at all stages of pre...
متن کاملThe Effect Of Vero Cells on Preimplantation Mouse Embryo in Cultur Medium
SUMMARY To study the effect of vero cells on preimplantation mouse embryos two groups of experiment were carried out. In experiment 1, pronuclear stage mouse embryos were co-cultured with Vero cells in RPMI medium and were examined under microscope for 4 days with 24h interval. The results showed co-cultured group developed better compared to control (87% to 80%, P<0.05) although, the rate ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Fertility and Sterility
سال: 2019
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2019.07.445